不同浓度的甘油蔗糖保护剂对人类精子冻存效果的研究

A STUDY OF THE EFFECTS OF PROTECTANTS WITH DIFFERENT CONCENTRATION OF GLYCEROL AND SUCROSE ON CRYOPRESERVATION OF HUMAN SPERMATOZOA

本文比较分析了4种浓度的甘油(0％、2.5％、5％、7％)与4种浓度的蔗糖(0、25、50、100mmol/L)组成的16种冷冻保护液在精子快速冻存时,对精子活动性的影响,观察精子快速冻存时蔗糖对精子活动性的影响。结果显示,添加蔗糖后的甘油保护液,较传统的甘油保护液能更有效地冻存精子,而且适当地增加蔗糖浓度、降低甘油浓度,可改善复温后的精子活动性,尤其是对保存精子的直线运动能力效果显著。用5％甘油与50mmol/L蔗糖作保护剂,冷冻复温后0、6、12小时时的精子存活率,分别为85.38％、51.22％、33.38％;直线运动精子存活率分别为83.74％、33.33％、18.38％。

The effects on frozen-thawed sperm survival rate of 16 different cryoprotective media containing different ratio of glycerol (0%, 2.5%, 5% and 7%) and sucrose (0,25,50 and 100 mmol/L) were compared. The results showed that glycerol-sucrose cryoprotective media had better effect on cryoprotection of spermatozoa than that of traditional glycerol protective medium, and appropriately increasing the concentration of sucrose and decreasing the concentration of glycerol could improve sperm motility, and especially benefit to preserve sperm linear motility at 12h postthawed. Using 5% glycerol combined with 50 mmol/L sucrose as cryoprotective medium, the sperm survival rate at 0, 6, 12h postthawed was 85.38%, 51.22%, 33.38%, respectively, the linear moving sperm survival rate was 83.74%, 33.33%, 18.38% respectively.