大鼠胃肠道的嗜银细胞——免疫组织化学和银浸技术的对比研究

THE ARGYROPHIL CELLS IN RAT GASTROINTESTINAL TRACT——A COMPARATIVE STUDY BETWEEN IMMUNOHISTOCHEMICAL AND SLIVER IMPREGNATION TECHNIQUES

采用在同一切片上,行免疫胶体金染色(IGS)与Grimelius嗜银染色的联合染色方法,对1O只大鼠胃肠道内的内分泌细胞与嗜银细胞进行了对比研究。结果表明,Grimelius嗜银染色法不能显示胃肠道的生长抑素细胞(SS细胞),也基本上不能显示胃泌素细胞(G细胞)。但除胃体外,Grimelius嗜银染色法在胃窦和肠道各段中基本上可用于显示肠嗜铬细胞(EC细胞)。此外,本方法还较其他方法有操作步骤少、结果可靠等优点,可以推广和应用。

The aim of this study is to investigate comparatively the relationship between the endocrine and argyrophil cells in the rat gastrointestinal tract by means of the IGS, PAP immunohistochemical and Grimelius technique in the same sections. The results showed that the SS cells and almost G ceils can not be identified by Grimelius technique. The Grimelius technique had high specifity to reveal EC cells in the antrum of stomach and the intestine. Our technique is relatively simple and reliable, and may be used for consecutive demonstration of argyrophil cells and bioactive peptide-containing cells.