温热合并吐温80对BGC-823人胃癌细胞超微结构和琥珀酸脱氢酶活性的影响

EFFECT OF HYPERTHERMIA IN COMBINATION WITH TWEEN 80 ON ULTRASTRUCTURE AND SDH ACTIVITY OF HUMAN STOMACH CANCER CELL LINE BGC-823

BGC-823人胃癌细胞系经吐温80合并温热(39℃～43℃)作用20～100分钟后,观察即时至96小时细胞超微结构和琥珀酸脱氢酶(SDH)活性的变化,并用正常人胚肺成纤维细胞作为对照。结果显示温热对细胞的作用,主要表现为粗面内质网扩张,线粒体肿胀、嵴减少,脂滴增多,SDH活性下降。43℃作用后,大量线粒体空泡化或髓样变.多聚核糖体减少,核异染色质增多,核仁呈块状或环形,部分细胞崩解坏死。吐温80单独作用与温热相似,与温热合并有明显协同作用,膜结构损害较早并更为严重,与41℃合并作用100分钟,已达到43℃100分钟温热效果,比单独使用温热可降低作用温度2℃左右。提示吐温80通过降低细胞相变温度,而加强温热对肿瘤细胞的杀伤作用。人胚肺成纤维细胞对合并作用反应较轻且易恢复。

Human stomach cancer cell line BGC-823 was treated by Tween 80 in combination with hyperthermia 39℃ to 43℃ for 20 to 100 minutes, respectively. The cell ultrastructure and succinate dehydrogenase (SDH) activity were studied at 0 hr, 48hr and 96hr after treatment respectively. Normal human fibroblast was used as control. The main changes of BGC-823 cells induced by hyperthermia were: dilation of endoplasmic reticulum, swelling of mitochondria, increase of fat droplets and decrease in SDH activity. After treatment at 43℃, the mitochondria showed vesicles or myelin figures and the polyribosomes tended to disaggregate. In nuclei, heterochromatin increased and the nucleolus become a dense homogeneous spot or annular ring. A few cells got necrosis. Effects of Tween 80 on BGC-823 cells were similar to that of hyperthermia. When Tween 80 combined with heating, marked synergic action was observed and the damage of membrane structure appeared earlier and more seriously. The effects of 41℃ combined with Tween 80 for 100 minutes were similar to that of 43℃ for 100 minutes alone. The critical temperature of hyperthermia for BGC-823 cells decreased to 41℃. The responses of the human fetal lung fibroblasts were slight and reversible. The results mentioned above suggested that the Tveen 80 enhanced the damage effect of hyperthermia on the tumor cells by decreasing the phase transition temperature of the tumor cells.