摘要
取DBA/2近交系小鼠活体骨髓细胞和小鼠胚皮肤(或肺组织)培养细胞,制备染色体标本。以GTG方法分带,可得到分散充分、分带清晰的早中期至中中期的分裂相。核型中可识别的带纹达435条。在大量核型分析基础上,绘制出DBA/2小鼠G-带核型模式图。本文描述了各号染色体G-带带型(纹)特征,并提出了某些带型相似染色体的鉴别要点。
Bone marrow cells and cultures of embryo skin and lung cells from DBA/2 mice were obtained for chromosome analysis in our studies. The specimens were banded by Giemsa staining following trysinization, which produced well-scattered and sharply banded mitotic figures ranging from early metaphase to mid-metaphase. Over 435 bands Within the mouse karyotype can be distinguished. Idiogram of G-banding patterns were constructed on the basis of large amount of karyotype analysis. The features of banding patterns of the individual chromosomes are presented and those chromosomes with similar banding patterns are contrasted to avoid possible misidentification.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第2期173-180,共8页
Acta Anatomica Sinica
