成年大鼠脊髓背角神经元受损轴突在外周神经移植物中的再生速度

RATE OF REGENERATION OF DAMAGED SPINAL AXONS INTO PERIPHERAL NERVE GRAFTS IN ADULT RAT

用抗神经丝(anti-neurofilament)单克隆抗体免疫组织化学技术,研究大鼠脊髓背角神经元受损轴突在外周神经移植物中的再生速度,发现脊髓背角神经元受损轴突在外周神经移植物中的最短初始延搁时间为4d;其后在一定时期内不断有再生神经纤维长入移植物,即不同神经纤维初始延搁时间不一致;再生轴突在移植物中的生长速度也不一致,最快再生速度为2.14mm/d。

Cho and So studied, with horseradish peroxidase retrograde tracing technique, the initial delay time and the rate of regrowth of damaged retinal ganglion cell axons regenerating into the autologous sciatic nerve implanted into the retinae in adult hamsters. This is the only report, to our knowledge, on the rate of regeneration of damaged central neuron axons. The present experiment tackles this issue using autologous sciatic nerve transplantation into the dorsal horn of the damaged spinal cord in adult rats, a model introduced by David and Aguayo, and visualized the regenerating axons with anti-neurofilament monoclonal antibody immunohistochemical method. Our results are as follows: the minimum initial delay time of the regenerating spinal axons in peripheral nerve grafts is 4 days. After which axons continue to regrow into the grafts within a definite period, suggesting different initial delay time for different regenerating axons. The regenerating spinal axons differ in their rate of regrowth, the fastest rate being 2.14 mm/d.