犬窦房结细胞质膜膜内微粒的形态测量——冷冻复型法

THE MORPHOMETRY OF THE INTRAMEMBRANOUS PARTICLES OF THE DOG SINUS NODE CELLS——FREEZE REPLICA METHOD STUDY

用冷冻复型法,对6只成体犬心脏窦房结的P细胞和移行细胞质膜膜内微粒(IMP),用Kontron opton IBAS Ⅰ+Ⅱ型图象分析仪处理,经换算获得下例各项参数。P细胞质膜PF面的微粒面积平均值为25.800nm^2,EF面者为17.816nm^2;移行细胞PF面膜内微粒的平均值为46.041nm^2,EF面者为88.633nm^2。P细胞质膜PF面IMP的周长平均值为20.735nm,EF面者为18.078nm;移行细胞质膜PF面IMP的平均值为28.780nm,EF面者为43.901nm。P细胞质膜PF面的IMP等面积圆直径平均值为5.220nm,EF面者为4.402nm;移行细胞质膜PF面IMP的平均值为7.010nm,EF面者为10.476nm。P细胞质膜PF面IMP的形态因子平均值为0.653,EF面者为0.643;移行细胞质膜PF面的IMP平均值为0.627,EF面者为0.573。P细胞质膜PF面密度为1325/μm^2,EF面为345/μm^2;移行细胞质膜PF面为3225/μm^2,EF面为590/μm^2。本研究对P细胞和移行细胞质膜PF面和EF面的各项参数进行了比较,并讨论了IMP的意义。

The morphometry of the intramembranous particles (IMP) of the dog sinus node cells was studied with freeze-cleaved specimens under H-500 electron microscope. The photographs of IMP of the P cells and transitional cells were analysed with imaging analytical system (IBAS Ⅰ+Ⅱ type). The results are as follows. The mean area of IMP: on PF and EF faces of P cells are 25.800nm^2 and 17.816nm^2. The mean values of IMP on PF and EF faces of the transitional cells are 46.041nm^2 and 88.633nm^2. The averages of the perimeter of IMP on the PF and EF faces of P cells are 20.735 nm and 18.078 nm, respectively. And those of the transitional cells are 28.780 nm and 43.901 nm. The diameters of circles of IMP of PF and EF faces of P cells are 5.220 nm and 4.402 nm. Those of PF and EF faces of the transitional cells are 7.010 nm and 10.476 nm. The mean values of form factor of IMP on PF and EF faces of P cells are 0.653 and 0.643, and those of transitional cells are 0.627 and 0.573. The mumbers of IMP are 1325/μm^2 and 345/μm^2 on PF and EF faces of P cells, and the mumbers of IMP of the transitional cells are 3225/μm^2 on PF face and 590/μm^2 on EF face.