大鼠顶盖提取液对神经细胞的诱向效应及其年龄性变化

NEURONOTROPHIC EFFECT OF TECTAL EXTRACTS FROM RATS OF DIFFERENT AGES

新生大鼠视网膜的分离细胞,培养在包被基板蛋白的96孔培养板作为细胞检测系统。用四唑盐MTT微量自动比色法定量不同浓度新生大鼠Te和出生后不同龄(0～12天)大鼠Te对视网膜细胞存活生长的影响。结果发现,新生大鼠Te对视网膜细胞存活生长的促进效应,随培养中Te浓度的增加而增强,当Te浓度增至15μ1/孔(约40ng蛋白)时,其效应达到饱和。出生后0～2天大鼠Te对视网膜细胞存活生长的促进效应最强,但随年龄增加则逐渐减弱,到出生后12天的大鼠Te,对视网膜细胞的效应与无Te对照组无显著差别(P>0.05)。提示大鼠Te对神经细胞的诱向效应与年龄性变化有关。

Dissociated neonatal rat retinal cells as testing cells were cultured on 96 well culture plates precoated with laminin. MTT colorimetric microassay was used to quantify the effect of various concentrations of tectal extracts (Te) from neonatal rats of defferent age (postnatal day 0-12) on the survival growth of retinal cells. It was found that the survival and growth enhancing effect of Te on the retinal cells increased with increasing Te concentration with a maximal effect achieved at 15 ul/well (about 40 ng protein). The survival and growth enhancing effect of Te from postnatal day 0-2 on the retinal cells was maximum. With further development there was a decline in the survival and growth enhancing effect of the Te. By postnatal day 12, the survival and growth enhancing effect of Te on the neonatal rat retinal cells had declined to a minimum with no difference from cultures without Te (p>0,05). The results suggest that the neuronotrophic effect of Te is changing With neonatal age.