大川芎方提取物对神经胶质细胞5-HT_(1D)受体的影响

The effect of Da Chuanxiong Fang extract on 5-HT_(1D) receptor expression of neurogila

目的 探讨大川芎方提取物是否影响神经胶质细胞 5- HT1 D受体。方法 体外培养人神经胶质细胞中给予1～ 3# 被试大川芎配方药的提取物 (1 0 %浓度 ) ,剂量 (ml)分别为 0 .0 6、0 .1 2、0 .2 4 ,作用 0 .5 h后加入特异性的抗体 sc-5394,用 FCM(流式细胞仪 )检测受体的表达 ,并用同样的方法检测阳性对照物川芎嗪注射液对 5- HT1 D受体的影响。结果各种不同比例配伍药物的提取物均可使荧光强度增强 ,即 :引起 5- HT1 D受体表达增加。结论 大川芎方可引起 5- HT1 D受体表达增加 ,作为 5- HT1 D受体的激动剂应用于偏头痛 。

Objective To investigate the influence of Da Chuanxiong Fang extract on 5 HT 1D receptor expression of neurogila. Methods Through biocytoculture in vitro, the neurogila cells of human were grown in a humidified incubator at 37 ℃ and 5%CO 2, and the examined drugs from 1～3#, tetramethypyrazine (10% concentration) and 0.9% NaCl were added into the cells. The dosages were 0.06, 0.12, 0.24 ml, respectively. After 0.5 hours, we put the antibody sc 5394 into cells to label them and check the fluorescene intensity with FCM. Results All of these drugs could increase the fluorescence intensity, and the action of 4# was the best in all of the examined drugs. Conclusion Da Chuanxiong Fang extract can influence the expression of 5 HT 1D receptor.