水貂初级卵母细胞发育过程中细胞器变化的电镜观察

ELECTRON MICROSCOPIC OBSERVATIONS ON CHANGE OF CYTOPLASMIC ORGANELLESIN THE DEVELOPMENT OF PRIMARY OOCYTES OF MINK

本实验将水貂卵巢内卵母细胞发育过程分成四个阶段,在卵母细胞恢复生长之前的第Ⅰ阶段,线粒体大而圆,缺乏嵴,卵母细胞恢复生长之后,许多线粒体变细变长,体积缩小,嵴增加,在第Ⅱ阶段晚期线粒体交圆,嵴减少,随着卵母细胞的发育,线粒体数量增加并逐渐移向皮层区.高尔基复合体始终位于质膜下的皮层区,粗面内质网罕见,滑面内质网不发达,以单个囊池形式存在,游离核糖体丰富,在第Ⅰ阶段卵母细胞中可见到不活跃贮藏形式的核糖体集团及成群的黑色嗜锇颗粒,卵母细胞恢复生长后消失,皮质颗粒出现,其数量不断增加,它们始终位于质膜下呈线状分布也是水貂卵母细胞的特点之一.在卵母细胞发育晚期,胞质中有大量脂滴.

The oocyte development was divided into four stages in this experiment.Large and round mitochon-dria with few or no arch-like Crista exist in the stage I before the resump-tion of oocyte growth.After that many mitochondria become Slender and longer with more Cristae,bnt the Volume decrease.At the late stage Ⅳ mitochondria become round and the number of Cristae decreases.The number of mitochondrion increases and they move to the cortical region with the oocyte development,Golgi com- plex is always located in the cortical region beneath the oolemma at all stages.Very few rough endoplasmic reticulum can be observed.Smooth endoplasmic reticulum is sinple in structure and small in number and often in single cistern form.Free ribosome particles are abundant.It is seen groups of ribosome Particles in inactive and store form and black oosmiophilic granules in groups.The number of cortical granules increase with the oocyte development and they always arrange themselves in line benea the oolemma which is also a characteristic of mink oocyte.Ooplasm contains a lot of lipid drops in mature oocyte.