纳米基因芯片在微生物学分子诊断中的应用

Applications of Nanogen Microelectronic Array Chip in Clinical Microbiological Molecular Diagnosis

开发纳米基因芯片的目的 ,就是将其用于微生物学分子诊断。纳米基因芯片技术具有广泛的用途 ,通过未知的带电生物分子特异性地与芯片上的已知分子结合 ,从而对其进行分析。采用电导分子杂交和基因控制技术在基因芯片上分析 DNA多态性或基因突变 ,可在同一基板上精确、迅速地同时进行几种测试。本文叙述纳米基因芯片技术在分子诊断测试中的广泛应用 ,如鉴定病原菌、细菌耐药性和细菌分型等 ,以及在研究传染病病原菌的作用。

Nanogen microelectronic array chip has been developed for molecular diagnosis in microbiology. Nanogen technology has broad applicstions for the analysis of unknown charged biological molecules that are capable of binding specifically to a known capture molecule on a microchip. Electronically mediated active hybridization and electronic stringency makes that DNA SNPs or gene mutations are easily analyzed on the microarray. The technique is rapid, sensitive and accurate and capable to perform several analyses on same platform at same time. A number of molecular diagnostic assays with Nanogen technology have been described in this paper. This technique has been successfully used in identification of bacterial pathogens, bacterial antimicrobial resistance, and bacterial genotyping. Nanogen technology will have great potential to be used for investigating the interaction between infections pathogens and human genomic defect, and vaccine research.