大鼠实验性脾虚证十二指肠粘膜内分泌细胞的免疫组织化学研究

IMMUNOHISTOCHMICAL STUDY ON THE ENTEROENDOCRINE CELLS OF DUODENUM MUCOSA IN "EXPERIMENTAL SPLEEN DIFICIENCY SYNDROME" IN RATS

用正常成年雄性Wistar大鼠46只,分为正常对照组,实验性脾虚组,自然恢复组和四君子汤治疗组.取十二指肠,Bouin液固定,制石蜡切片,进行了以下染色.(1)H.E染色(2)按免疫组织化学PAP法显示粘膜的胃泌素(G)细胞、生长抑素(D)细胞和5-HT细胞.在光镜下按照半定量法将细胞分为强阳性、中等阳性和弱阳性.观察并记录每组动物切片中各级活性细胞数和计算各级细胞的百分比.(3)从正常对照组,脾虚组各随机选择5例动物,对D细胞和5-HT细胞进行显微分光光度计的定量测定.结果表明(1)脾虚组粘膜未见明显的组织学变化.(2)内分泌细胞:与对照组相比,脾虚组G细胞、5-HT细胞内反应物含量减少,表明分泌功能增强.D细胞内反应物含量增多,表明分泌功能减弱.统计学分析表明以上结果有显著性差异(P值<0.01).与自然恢复比较,治疗组接近对照组.本文结果提示,脾虚证的发病与消化道内分泌细胞变化有密切关系;四君子汤对脾虚证有较好的治疗作用.

46 adult Wistar male rats were used and divided into 4 groups,i.e.normal control group, 'experi-mental spleen dificiency group'induced by rhubarb,spontaneous recovery group and therapeutic group treated with Chinese herbs (Si Jun Zi decoction).All the animals of the 4 groups were killed and processed for demonstration of gastrin cells (G cells).Somatostatin cells(D cells) and 5-HT cells according to immunohistochemical PAP technique.The number of strong positive(+++),moderate positive (++) and weak positive (+) cells of these three types was counted and the percentage of positive cells of each grade wer calculated.In addition,the immunoreactivities of D cells and 5-HT cells in nomal control groups and 'experimental spleen dificiency group' were measured by microspectrophotometer (MPV-2,Leitz).All the data were treated statistically.The percentage of G cells(++),5-HT cells(+),D cells(++,+++) in 'the experimental spleen dificiency group' was more than that of the normal control group,while the percentage of G cells(+++),5-HT cells(++,+++),D cells(+) was less than that of the normal control group.The both groups showed obvious difference(P<0.01).As compared with spontaneous recovery group the percentage of G cells,5-HT cells and D cells in the theraputic group was approxi- mated to the normal control group. The immunoreactivities of D cells and 5-HT cells measured with microspectrophoter appeared similar to the semiquantitatixe results.Our study suggested that the changes of percentage of G cells,5-HT cells and D cells in duodenum mucosa may be closely related to digestiv disorders presented in spleen dificiency syndrome,and the Chinese herbs(Si Jun Zidecoction) currently used possessed significant therapeutic effect.