儿茶素对肾病综合征大鼠系膜细胞增生的影响

研究儿茶素对肾病综合征大鼠肾小球系膜细胞增生的影响,阐明儿茶素对肾病综合征大鼠疗效的可能机制。方法:20只SD(Sprague-Dawley)雌性大鼠随机分成对照组、肾病组、儿茶素预防组、儿茶素治疗组共四组。实验末应用生物化学法测定血清中白蛋白(Albumin, Alb)、总蛋白(Total protein, TP)、三酰甘油(Trilyceride, TG)、血尿素氮(Blood Urea Nitrogen, BUN)、及24h尿蛋白排泄量(24hrs urinary protein,24hrsUP),应用免疫组化检测肾系膜中细胞周期调控蛋白P21、P27、PCNA、CyclinD1及TGF-β1的表达水平。结果各组大鼠肾小球系膜细胞CyclinD1、PCNA、P21、TGF-β1表达水平较对照组明显增高(p均<0.01),P27水平明显降低(p<0.01)。与肾病组相比,儿茶素预防组和治疗组系膜细胞P21、PCNA、CyclinD1、TGF-β1表达水平明显降低(p均<0.01),而P27表达水平增高明显(p<0.01)。儿茶素预防组和儿茶素治疗组无明显差异。肾病组大鼠尿蛋白排泄量较对照组显著增加(p<0.01)。从血清白蛋白浓度来看,儿茶素预防组、儿茶素治疗组明显高于肾病组,而血清TG则明显降低(p均<0.01),BUN也略低于肾病组,以儿茶素预防组较明显。儿茶素预防组和儿茶素治疗组血清总蛋白水平较肾病组有显著增加(p<0.05)。结论:儿茶素通过抑制P21、PCNA。

To study the effect of catechin on glomerular mesangial cells proliferation in rats nephrotic syndrome to elucidatethe related influence on nephrotic syndrome.Methods:20 female SD rats were randomly divided into four groups:control,nephrotic, catechin-prevented and catechin-treated.In the end of experiment, Alb. TP,TG,BUN in serum and 24hr urinaryprotein were detected by biochemical assay. The expressions of P21,P27,PCNA,CyclinD1,TGF-β1 related cell cycle regulatoryprotein in renal mesangial cells were measured by means of immunohistochemical technique. Results:compared with controlgroup,the related cell cycle regulatory proteins as P21,PCNA,CyclinD1,TGF-β1 expressions in glomerular mesangial cells werehigher in the other groups (all p<0.01),while P27 expression was lower(p<0.01).Controlled with nephrotic group, theexpressions of P21,PCNA,CyclinD1,TGF-β1 in catechin-prevented and catechin-treated group were significantly lower(all p<0.01),but P27 expression was obviously higher (p<0.01).There was no significant difference between catechin-prevented andcatechin-treated group. Urinary protein extraction by rats in nephritic group increased more than control group(p<0.01).As tothe concentration of Alb in serum, the catechin-prevented and catechin-treated groups were higher than nephrotic group,Theconcentration of TG also significantly decreased (p<0.01).BUN was a little lower than nephrotic group,especially in catechin-prevented group.The concentrations of TP in catechin-prevented and catechin-treated group higher than nephrotic group (p<0.05).Conclusion:catechin could inhibit the proliferation of glomerular mesangial cells, maintain glomerular permeability,de-crease the extraction of urinary protein,improve normal lipid metabolism,recover normal renal function and slow chronicprogressive kidney lesions through improving P27 expression and decreasing P21、PCNA、Cyclind1、TGF-β1 inglomerular messangial cells.