植酸钙的提取及固定化细胞植酸酶酶解

本文重点研究了以麦麸为原料提取植酸钙的工艺条件,最佳工艺条件为60℃水浴浸泡6h,通过浸泡方式的改进,以二次浸泡和动态摇床浸泡两种方式进行实验,结果发现这两种方式均使植酸钙得率提高。另外,利用在细胞壁表面产植酸酶的克鲁维假丝酵母,采用细胞固定化技术,进行植酸酶酶解。细胞固定化和酶解反应的方法都比较简单,而且多次反应后酶解率损失较低。此法为肌醇、磷酸肌醇的酶法制取提供了可行性方案。

This paper mainly discussed how to extract calcium phytate with bran. The best condition of the experiment wasto soak the bran in water at 60℃ for 6h. The second soak or dynamic state vibration in a rocking bed could both increase the extractrate of calcium phytate.In addition, it was a novel way to use the phytase produced by the Kluyveromyces yeast on the cell wallsurface to hydrolyze the wheat bran with the technology of immobilized cell. Both the immobilization of cell and the way ofhydrolysis were easy to operate, and the loss of hydrolysis was not much after many times of extraction. This provided a feasibleway to produce the calcium phytate.