粘菌基因组DNA提取方法的研究

Studies on Genomic DNA Extraction of Myxomycetes

针对粘菌标本(孢子)数量少、形态微小等特点,分别采用CTAB法、SDS法、蛋白酶K法4和SDS-蛋白酶K4种方法,提取基因组DNA。所得DNA完整,可用于RAPD等研究。

Considering the fact that the specimens of myxomycetes are small in amount and minute in form, four methods, CTAB method, SDS method, protease K method and SDS - protease K method, are used to extract genomic DNA. The DNA is intact and can be used in RAPD study and other molecular researches .