中外11个猪种H-FABP基因PCR-RFLP的研究

Study on the Polymorphism of Porcine Heart Fatty Acid-Binding Protein Gene in Eleven Pig Breeds Using PCR-RFLP

本文利用PCR RFLP技术对五指山猪、沂蒙黑猪、汉江黑猪、莱芜猪、香猪、民猪、成华猪、内江猪、二花脸猪、巴马香猪和大白猪11个猪种共420头猪的心脏脂肪酸结合蛋白基因5′ 端上游区(HinfI RFLP)和第二内含子内(HinfI RFLP、HaeⅢ RFLP)的遗传变异进行了研究。研究表明:(1)在5′ 端上游区的HinfI RFLP位点上,所有的品种都有变异,但沂蒙黑猪和大白猪只有两种基因型(HH、Hh);(2)在第二内含子内的HinfI RFLP位点上,二花脸只有BB基因型,而其它品种都表现出多态性;(3)在第二内含子内的HaeⅢ RFLP位点上,只在五指山猪、香猪和大白猪中发现有多态性存在,等位基因D的频率分别为0 86,0 98和0 68,其余8个猪种均表现为DD型。

The genetic variation in 5′upstream (HinfⅠRFLP) and in intron 2 (HinfⅠRFLP?HaeⅢRFLP) of the porcine heart fatty acidbinding protein (HFABP) gene were detected with PCRRFLP in 420 pigs including Wuzhishan pig,Yimeng black pig,Hanjiang black pig,Laiwu pig,Xiang pig,Min pig,Chenghua pig,Neijiang pig,Erhualian pig,Bama xiang pig and Large White pig.The results suggested:(1)All the breeds varied at the HinfⅠRFLP site of the 5′upstream while Yimeng black pig and Large White only had 2 genotypes (HH?Hh).(2)At the HinfⅠRFLP site of the second intron,Erhualian only presented BB genotype while other breeds varied.(3)At the HaeⅢRFLP site of the second intron,the polymorphic site was only found in Wuzhishan,Xiang pig and Large White,D allele frequency was 086,098 and 068,respectively,other 8 breeds only showed DD genotype.