氟苯尼考和中草药黄芪淫羊藿合剂对鸡体液免疫反应的影响

The Effects of Florfenicol and Chinese Herbal Ingredients Radix Astragali and Herba Epimedii on Humoral Immune Response in Chicks

试验分别对免疫期间的各组鸡连续两周给予不同剂量的氟苯尼考、黄芪淫羊藿合剂后,在不同日龄采取血样,用血凝抑制(HI)试验分别对鸡新城疫(ND)抗体水平和禽流感(AI)抗体水平进行监测。结果表明,混饮给药,小剂量(50mg/L)氟苯尼考对ND抗体水平影响不显著(P>0 05),而中(150mg/L)、高(250mg/L)剂量氟苯尼考对ND抗体产生有显著的抑制作用(P<0 01);氟苯尼考追加给药1周后,中、高剂量组ND抗体水平又有显著降低(P<0 05),而小剂量组ND抗体水平差异不显著;中(15ml/L)、高(22 5ml/L)剂量黄芪淫羊藿合剂对ND、AI抗体产生有显著增强作用(P<0 01);对因氟苯尼考造成的免疫抑制,黄芪淫羊藿合剂可使抗体水平恢复至对照组水平(P>0 05)。

Chicks were administered florfenicol,ingredients Radix Astragadli and Herba Epimedii of different dosages orally for two weeks during inoculating period for investigating the effects of the drugs on antibody production.Blood samples were collected in different time after oral administration.The Newcastle disease(ND) and the Avian Influenza (AI)antibody titers were determined by hemagglutination inhibition(HI) test respectively.The results indicated that no significantly lower response was measured in chicks that received florfenicol at a low dosage of 50 mg/L drinking water(P>005),whereas ND antibody titers were significantly decreased in chicks that received the drug at a middle dosage of 150 mg/L drinking water and a high dosage of 250 mg/L drinking water(P<001).ND antibody titers were significantly decreased in chicks that were additionally administered florfenicol for a week at a middle dosage and a high dosage (P<005),while no significantly decreased response was observed in chicks that were administered florfenicol at low dosage(P>005).ND and AI antibody titers were significantly higher by drinking ingredients Radix Astragali and Herba Epimedii at middle(15 ml/L drinking water)and high (225 ml/L drinking water)dose (P<001),as compared with the control group.The humoral immune response impairment caused by florfenicol could be controled and the antibody titers could be adjusted to control level by using ingredients Radix Astragali and Herba Epimedii(P>005).