鹅Ⅰ型禽副黏病毒F基因重组质粒的构建及其DNA免疫

Construction of recombinant plasmid containing the F gene of GPMV/QY97-1 strain of goose's paramyxovirus-1

通过RT PCR扩增出鹅Ⅰ型禽副黏病毒GPMV/QY97 1株F蛋白基因的cDNA ,并将F基因cDNA克隆到含CMV启动子的pCI载体上 ,构建这一基因的真核表达质粒pC GPMVF ,以此质粒肌肉注射 3周龄非免疫鸡 ,并分别于免疫后第 2、4、6周通过ELISA方法检测抗体水平。结果证明 ,用重组质粒pC GPMVF免疫鸡 。

The fusion protein (F) of the avian paramyxovirus 1 is the major inducer of the generation of protective immune response against virulent virus challenge. On the basis of these observations, the full length cDNA of fusion protein (F) gene of the goose's paramyxovirus 1 strain GPMV/QY97 1 was amplified by RT PCR. The recombinant plasmid vector (pC GPMVF)containing the fusion protein (F) gene under the control of the human cytomegalovirus (hCMV) immediate early enhancer was constructed and its immunogenicity were evaluated. After 3 week old non immunized chickensinjected intramusculary with plasmidpC GMVFDNA, the antibody against avianparamyxovirus 1 was detected by ELISA.Theresults demonstrated that the recombinant plasmid pC GPMVF can elicit antibody against avianparamyxovirus 1, and exhibit an anti avianparamyxovirus 1 humoral immune response.