二氧化硒诱导肺腺癌SPCA-1细胞凋亡的研究

Induction of apoptosis in human lung carcinoma SPCA-1 cell line by selenium dioxide(SeO_2)

目的 :研究二氧化硒诱导肺腺癌细胞系SPCA - 1细胞凋亡。方法 :采用台盼兰拒染法和四甲基偶氮唑蓝 (MTT)比色法测定不同浓度二氧化硒作用不同时间对肺癌SPCA - 1细胞生长的抑制作用 ;采用流式细胞 (FCM)技术 ,观察二氧化硒作用后SP CA - 1细胞的凋亡率及细胞周期的变化 ;通过HE染色和透射电镜观察SPCA - 1细胞的形态变化。结果 :二氧化硒可有效的地抑制SPCA - 1细胞的生长 ,具有时间、浓度依赖性 ;二氧化硒诱导SPCA - 1细胞的凋亡具有浓度依赖性 ;二氧化硒低浓度时阻滞SPCA - 1细胞于S期 ,高浓度时选择性诱导S期细胞凋亡 ;二氧化硒作用后 ,SPCA - 1细胞呈现典型的凋亡细胞形态特征。结论 :二氧化硒通过诱导S期细胞凋亡而抑制肺腺癌细胞系SPCA - 1细胞的生长 ,具有时效。

Objective To study apoptosis of lung carcinoma SPCA-1 cell line induced by SeO 2. Methods: Trypan blue dying and Methyl thiazolyl tetrazolium(MTT) was used to observe the growth inhibition rates of SPCA-1 cells by various concentration of SeO 2 at different time ponts. Apoptosis percentage and cell cycle phase distribution of SPCA-1 were measured by flow cytometry (FCM) assays. Apoptotic phenotype of SPCA-1 was observed by HE dying and electric microscope . Results: SeO 2 could inhibit the growth of SPCA-1 cells effectively and depended on the action time and concentration of the medicine. SPCA-1 cell apoptotic percentage induced by SeO 2 was enhanced in concentration-dependent pattern. S phase cell of SPCA-1 were arrested in lower concentration of SeO 2, while higher concentration induced apoptosis selectively. SPCA-1 cells treated by SeO 2 showed morphological characteristic of apoptotic cells. Conclusion: SeO 2 can inhibit the proliferation of lung carcinoma SPCA-1 cells by inducing S phase cells apoptosis, and it is time-dependent and concentration-dependent and cell cycle specific.