氟伐他汀预防大鼠肺肿瘤的实验研究

Experimental study of fluvastatin to prevent rats from the development of lung tumors

目的 :探讨氟伐他汀预防肺肿瘤的作用。方法 :SD大鼠 36只随机分对照组 (A组 )、氟伐他汀B组及C组 ,每组 1 2只 ,B组及C组分别按 1 0mg·kg-1 及 2 0mg·kg-1 给药。在诱发肺肿瘤过程中观察外周血网织红细胞微核率的变化 ,98d后观察肺肿瘤形成及K -ras,p5 3蛋白表达情况。结果 :2 1d显示B组与C组网织红细胞微核率明显较对照组减少 ,分别为 (1 1 .6±6 .8)‰ ,(1 1 .7± 5 .7)‰及 (1 9.0± 9.2 )‰ ,P均 <0 .0 1。C组平均荷瘤鼠肿瘤数较对照组明显减少 ,分别为 (1 .0± 1 .3)及 (3.9± 3.5 ) ,P <0 .0 1 ,但B组与对照组无显著性差异。对照组肿瘤发生率为 75 % (9/ 1 2 ) ,1 0mg·kg-1 组为 6 7% (8/ 1 2 ) ,2 0mg·kg-1组为 5 0 % (6 / 1 2 ) ,但无统计学差异 (P >0 .0 5 )。三组荷瘤大鼠K -ras,p5 3表达率无明显差异 (P >0 .0 5 )。结论 :氟伐他汀能抑制肺肿瘤形成及其增殖 ,减少外周血网织红细胞微核率 ,表现出潜在的抗肿瘤作用 。

Objective: To investigate the protective effect of fluvastatin on lung tumor. Methods: Thirty-six SD rats were randomly divided into three groups and each of twelve, fluvastatin treatment group (group B and group C with different dosage) and control group (group A). Peripheral blood reticulocyte micronuclei frequence(PBRMNF) was measured in all rats during tumor-inducing procedure. Lung tumor development and K - ras and p53 expressions were observed 98 days later. Results: On the 21th day, compared with group A(19.0±9.2), PBRMNF in group B (11.6±6.8)and group C (11.7±5.7) were significantly decreased( P <0.01). Average tumor number per tumor-bearing rat was decreased in group C (1.0±1.3) much more than that in group A (3.9±3.5, P <0.01). However, no significant difference appeared between group A and group B ( P >0.05). The tumor incidences of three groups were 75%(9/12), 67%(8/12) and 50%(6/12) respectively, and among them, there were no any statistic differences( P >0.05). K - ras and p53 expressions of tumors in three groups had no significant difference( P >0.05).Conclusion:Flusvastatin can inhibit lung tumor development and reduce PBRMNF.