1,6-二磷酸果糖对大鼠缺血性心脑损伤的保护作用

Effccts of fructose-1,6-diphosphate on myocardial and cerebral lesion induced by isoprenaline in rats

目的 探讨口服 1,6 二磷酸果糖 (FDP)对大鼠缺血性心脑组织损伤的保护作用及其作用机制。方法 采用异丙肾上腺素 (Iso)致大鼠实验性心肌缺血模型。随机分为 3组 :对照组、Iso组 [5mg/(kg·d) ,7d]和Iso +FDP组 [Iso ,5mg/(kg·d) ,7d ;FDP ,6 g/(kg·d) ,2 1d],每组 8只。用光镜、电镜观察心、脑组织病理形态学改变。测定心肌肌浆网钙泵 (SERCA)活性、超氧化物歧化酶 (SOD)活性。结果 Iso组大鼠心肌组织病理可见大量纤维化病灶 ,病灶多呈片状、多灶性 ,还可见少许凝固坏死灶 ;亦可见脑组织病理损害、海马神经元密度较对照组降低。心肌SERCA活性 [0 .2 5 0± 0 .0 6 3μmol/(min·g·wetweight) ]较对照组 [0 .334± 0 .0 6 1μmol/(min·g·wetweight) ]降低。脑组织匀浆SOD活性 [2 3.2 2± 3.0 7U/(mg·pro) ]低于对照组 [4 4 .89± 2 .771U/(mg·pro) ]。FDP使心肌组织纤维化病灶减少 ,脑组织损害也明显减轻 ;FDP使心肌SERCA活性 [0 .312± 0 .0 6 7μmol/(min·g·wetweight) ]和SOD活性 [39.70± 3.4 4 1U/(mg·pro) ]增高。结论 口服FDP可减轻大鼠心肌和脑组织病理改变 。

Objective Effects of fructose l,6 diphosphate (FDP) on myocardial and cerebral lesions induced by isoprenaline (Iso) in rats were studied.Methods The models of myocardial ischemia were made by Iso treatment.Twenty four rats were randomly divided into 3 groups:control group ( n =8),Iso group ( n =8) [5 mg/(kg·d), 7 d], Iso+FDP group ( n =8) [Iso,5 mg/(kg·d), 7 d;FDP, 6 g/(kg·d),21 d].The pathologic changes in myocardial and cerebral tissues were observed by lightmicroscope and electron microscope. Levels of activity of sarco/endoplasmic Ca 2+ ATPase (SERCA) in the myocardium and superoxide dismdese (SOD) in the cerebral tissue were detected.Results In Iso treated rats, there were many fibrosis focal changes in myocardium including flat, multifocal, and coagulate necrosis. The density of hippocaxnpial neuron was lower compared with rats of control group. The activities of SERCA from myocardium and SOD from cerebral tissues were markedly decreased than those of control group. FDP reduced the pathological changes of myocardial and cerebral tissues in rats induced by Iso. Also, FDP enhanced the activity of SERCA from myocardium and SOD from cerebral tissue. Conclusion FDP has protective effect on myocardial and cerebral tissues reduce pathological changes in myocardial and cerebral tissue of rats induced by Iso.