杂多化合物九钨三钛硅酸盐的急性毒性和致突变性研究

STUDY ON ACUTE TOXICITY AND MUTAGENTICITY OF POLYOXOANIONS α-K_8H_2[SiW_9Ti_3O_40]·15H_2O

目的 :对杂多化合物九钨三钛硅酸盐(α -K8H2[SiW9Ti3O40]·15H2O ,简称α -Ti3)进行急性毒性和致突变性研究 ,为临床应用提供科学依据。方法 :采用小鼠经口急性毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验及CHO细胞染色体畸变试验等方法进行检测。结果 :①小鼠经口急性毒性试验 ,LD50 为2055.36mg/kg,属低毒物质 ;②α -Ti3 浓度在31.3～500μg/皿范围内 ,无论加S9 与否 ,每皿菌落回变数均未超过阴性对照组的两倍 ;③微核试验在25、50、125mg/kg3个剂量水平 ,各剂量组对小鼠骨髓嗜多染红细胞微核千分率均未见明显增加 ,与阴性对照组比较无显著性差异(P>0.05) ;④α -Ti3 浓度在40～320μg/ml范围内 ,无论加S9 与否 ,CHO染色体畸变率与阴性对照组比较无显著性差异(P>0.05)。结论 :杂多化合物九钨三钛硅酸盐毒性较低 ,在本试验条件下 ,诱变性试验结果为阴性。

Purpose: To study the oral acute toxicity and mutage ni city of Polyoxometalates α-K8H2[SiW9Ti3O40]·15H2O for clinic applica tion(α-Ti3). Methods: The studies were conducted with acute toxicity test,Ames test,micronuclei test and chromosome aberration test on CHO cell in vitro. Results: ① LD50 in mice was 2 055.36 mg/kg ,it was a low-grade toxicity compound .②The results of Ames test showed no mutagenic effects with th e concentration ranged from 31.3 to 500 μg/plate with or without S9 mixture added. ③In micronuclei test,the mice orally received 25,50,125 mg/kg level of α-Ti3,no mutagenic e ffects were observed,and no significant increase was observed on polychromatophil.④The chromosome aberration ratio of α-Ti 3 was no significant discrepancy compared with the control on CHO cells,with the concentration ranged from 40 to 320 μg /ml with or without S 9 mixture added. Conclusion: The t oxicity of α-Ti 3 was low and no mutagenicity was observed in the experiment.