蒲公英对环磷酰胺致小鼠骨髓细胞突变作用的抑制研究

THE EFFECT OF TARAXACUM MONGO TO INHIBIT THE MUTATION IN THE BONE MARROW LYMPHOCYTE OF MICE INDUCED BY CYCLOPHOSPHAMIDE

目的 :研究蒲公英(TM)抗突变作用和对细胞增殖的影响。 方法 :采用小鼠骨髓淋巴细胞有丝分裂指数试验、染色体畸变试验、微核试验 ,观察蒲公英水煎液对小鼠骨髓淋巴细胞的遗传损伤效应的影响。结果 :①环磷酰胺(CP)单独作用时 ,能抑制小鼠骨髓淋巴细胞的增殖(P<0.01) ;与TM联合作用时 ,有丝分裂指数明显比阳性对照组提高(P<0.01)。②蒲公英低、中、高剂量组均能明显地抑制由于CP引起的染色体畸变率 ,其抑制率分别为39.62 %、58.49 %、54.72 % ,同时对CP诱发的微核率也有明显的抑制效应 ;蒲公英水煎液不会导致小鼠骨髓淋巴细胞的染色体畸变和微核率的增加(P>0.05)。结论 :蒲公英本身没有致突变性 ,能抑制CP诱发的染色体畸变和微核率 ,促进细胞的增殖能力。

Purpose: To study the effects of Taraxacum mongo(T M) on anti-mutation and cell proliferation. Methods: Mitotic Inde x(MI), chromosome aberration and micronucleus test in bone marrow lymphocytes of mice were determined. Results: ①The MI of bone marrow lymphocytes in mice were in hibited by CP(P < 0.01),but MI were significantly increased compared with contr ol group after TM oral feeding on mice (P < 0.01). ②The in hibition rates of chromosome aberration were 39.62 %, 58.49 % and 54.72 % ,respectively by TM in different concentrations of low, medium and high, furthermore the frequencies of micronucleu s(MN) were inhibited by CP(P > 0.05). Conclusion: No mutagenici ty was observed on TM .but TM possesses the ability of anti _ mutation,and promotes cell proliferation.