正常昆明种与NIH种小鼠自然发生微核率的比较

A COMPARISON OF NATURAL MICRONUCLEUS FREQUENCIES IN POLYCHROMATIC ERYTHROCYTES BETWEEN KUNMING MICE AND NIH MICE

目的 :比较正常昆明种与NIH种小鼠胸骨骨髓嗜多染红细胞微核的自然发生率。 方法 :按《食品安全性毒理学评价程序和方法》采用30h法微核试验 ,计数微核数 ,计算微核率。 结果 :小鼠胸骨骨髓PCE自然发生率呈正偏态分布 ,中位数能较好的代表其平均水平 ,昆明种小鼠胸骨骨髓PCE微核自然发生率(‰) ,雌鼠为1.0(0,4.0) ,雄鼠为2.0(0 ,6.0) ,性别间差异有统计学意义(P<0.05) ;NIH种小鼠胸骨骨髓PCE微核数自然发生率(‰) ,雌鼠为2.0(0 ,3.0) ,雄鼠为2.0(0,6.0) ,性别间差异无统计学意义。昆明种和NIH种小鼠胸骨骨髓PCE自发微核率间差异无统计学意义。 结论 :昆明种与NIH种小鼠胸骨骨髓PCE微核的自然发生率与其他人的研究较接近 ,昆明种与NIH种小鼠品系间差异无统计学意义 ,而昆明种小鼠性别间差异有统计学意义。

Purpose: To compare natural micronucleus frequencies in polychromatic erythrocytes (fMPCE) from normal Kunming mouse ch est bone marrow to that from normal NIH strain. Methods: Acc ording to Procedures and methods for toxicological assessment on food safety, 30 h micronucleus test was performed, the numb ers of micronuclei were scored, and the fMPCE was calculated. Results: The natural fMPCE from the chest bone marrow showed skew distribution, and the media was a good indicator. The natural fMPCE of Kunming mice was 1.0 (0, 4.0) in female, a nd 2.0 (0, 6.0) in male, and there was a significant differe nce between different sex (P<0.05). The natural fMPCE of NIH mice was 2.0 (0, 4.0) in female, 2.0 (0, 6.0 ) in male, and there was no significant difference between d ifferent sex. There was no significant difference between Kunmin g and NIH mice in the natural fMPCE. Conclusion: The natural fMPCE from chest bone marrow of Kunming mice and NIH mice are similar. There is no significant difference between two strains; but a significant difference between different sex in Kunming mice does exist (P<0.05).