原癌基因C-jun在大鼠变应性鼻炎鼻黏膜炎症细胞的表达及相关性研究

Expression of oncogene C-jun in the cells of rat nasal mucosa and relevance with rhinorrhea

目的 :探讨原癌基因C jun在大鼠变应性鼻炎鼻黏膜中的表达及其在变应性鼻炎发病机制中的作用。方法 :将 4 0只大鼠随机分为实验组 (30只 )和对照组 (10只 ) ,实验组根据制模成功后的时间又分为 30min组、4h组和 8h组 ,每组 10只。以卵清蛋白为致敏原建立大鼠变应性鼻炎动物模型 ,制模成功后 ,分别测各组动物的鼻分泌量 ;并取大鼠鼻黏膜组织 ,应用免疫组化技术、组织学检查等方法 ,检测C jun在鼻黏膜组织中的表达及各组鼻黏膜组织的病理学改变 ;应用计算机图像分析技术 ,测定阳性细胞的灰度值 ,并对鼻分泌量与阳性细胞灰度值进行相关分析。结果 :C jun免疫反应阳性物质呈棕色 ,分布于上皮细胞及多形核炎症细胞的细胞核中。30min组 ,阳性细胞数最多 ,其平均灰度值与对照组相比 ,差异有极显著性意义 (P <0 .0 1) ,并与鼻分泌量呈正相关 (r =0 .784 ,P <0 .0 5 ) ;4h组 ,C jun在炎症细胞内表达减弱 ,但其灰度值与对照组相比 ,差异仍有极显著性意义 (P <0 .0 1) ,亦与鼻分泌量呈正相关 (r =0 .6 2 6 ,P <0 .0 5 ) ;8h组 ,C jun的表达与对照组相比 ,差异无显著性意义 (P >0 .0 5 ) ,且与鼻分泌量无相关性。结论 :原癌基因C jun的表达可能是变应性鼻炎中炎症细胞活化的早期标志 ,与炎症细胞的增殖、分化有着密?

Objective:To study the expression of oncogene C jun in rat nasal mucosa with allergic rhinitis and the relevance with rhinorrhea.Method:By using EA to make animal model of allergic rhinitis successfully; the amount of rhinorrhea was calculated at 30 min. 4 h, 8 h. Immunohistochenical technique, computer image analysis techique and histologic examination were used to investigated the expression of C jun in rat nasal mucosa at the same time. The relevance between them was used explored.Result:There was a lot of C jun protein at 30min. The difference of C jun between 30min group and control group was significant (P< 0.01 ) and the relevant coefficient was r= 0.784 (P< 0.05 ). The C jun protein decreased at 4 h, but there was still significant difference (P< 0.05 ) and r= 0.626 (P< 0.05 ). The C jun was as same as control group at 8 h, There was no difference between them.Conclusion:The expression of oncogene C jun in inflammatory cells may be a marker in early stage of activation of inflammatory cells, and related to the produciug and secreting of cytokines and inflammatony mediators in inflammatory cells of allergic rhinitis.