模拟失重及力负荷对大鼠胫骨骨液流的影响

Effects of Simulated Weightlessness and Mechanical Loading on Bone Interstitial Fluid Flow in Rats

目的采用体内分子示踪方法观察尾吊大鼠胫骨骨陷窝小管液流改变及施加力负荷后的变化。方法 36只SD雄性大鼠分 3组 :自由活动组 ,悬吊组 ,悬吊 +力负荷组。实验第 2 1天 ,每组选 4只大鼠尾静脉注射辣根过氧化物酶 (HRP)溶液 ,3h后断头处死大鼠 ,取胫骨冰冻切片 ,染色后观察HRP反应产物在骨组织的分布情况。结果悬吊组大鼠骨陷窝染色反应物沉积量明显少于自由对照组 ;力负荷组大鼠骨陷窝反应物沉积量明显多于悬吊组。结论模拟失重条件下 ,大鼠骨陷窝小管液体流动受到了一定程度的抑制 ,短时间一定强度的力负荷刺激 ,可促进其液体流动 。

Objective To investigate the effects of simulated weightlessness and mechanical loading on bone interstitial fluid flow. Method Thirty six male Spargue Danley rats were divided into 3 groups: the control group, the tail suspension group, and the tail suspension plus mechanical loading group,with four rats in each group.All the rats were injected via a lateral tail vein with horseradish peroxidase on the 21st day of the experiment.Tibial tissue specimens were explanted, fixed, decalcified and cut into 30 μm frozen sections 3 h after the intravenous injection. Result There were less peroxidase reaction product in the bony matrix and bone lacunae in tail suspended rats than the control and tail suspended plus mechanical loading rats. Conclusion Tail suspension decreased fluid movement through the bone, while mechanical loading increased it.