摘要
目的研究模拟失重骨质和骨髓内骨钙素 (OC)以及骨和软骨钙盐代谢的形态学变化。方法选用2 0只SD大鼠 ,随机配对分为 1 4d和 2 8d尾悬吊组及相应的 2个自由活动组 ,每组 5只。组织标本行原位杂交及三色染色。结果尾吊组大鼠骨质和骨髓内OC的表达明显弱于对照组 (P <0 .0 5 ) ;尾吊组1 4dOC的表达明显强于 2 8d(P <0 .0 5 )。模拟失重导致股骨和软骨基质钙化障碍 ,原有钙化基质内钙盐脱钙明显 ,2 8d脱钙明显加重。结论大鼠后肢去负荷导致骨和骨髓OC含量的降低 ,骨和软骨钙盐代谢障碍 。
Objective To study osteocakin(OC) changes in bone and marrow and calcium deposition in bone and cartilage under simulated weightlessness. Method Twenty SD rats were randomly divided into 14 d and 28 d tail suspension group and 2 corresponding control groups. Histological samples were in situ hybridized and trichrome stained. Result OC expression of bone and marrow of rats were lower in tail suspended rats than that in the control(P<0.05). OC expression in 14 d tail suspended rats were higher than that in 28 d tail suspended group(P<0.05). Mineralization was inhibited, and demineralization of furmer and cartilage mineralized matrix was prominent. Demineralization was more prominent in 28 d group.Conclusion OC levels in bone and marrow of rats were lower after tail suspension. Calcium deposition was inhibited in bone and cartilage. Demineralization was prominent after long term hindlimb unloading.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2003年第4期260-263,共4页
Space Medicine & Medical Engineering
基金
全军医药卫生科研基金资助项目 (98Z0 83 )
关键词
失重模拟
尾吊
骨钙素
骨
骨髓
原位杂交
weightlessness simulation
tail suspension
osteocalcin
bone
bone marrow
in situ hybridization
