HPLC法测定山东4种菟丝子槲皮素、山萘酚的含量

Determination of quercetin and kaempferol of four kinds of Semen Cuscutae in Shandong by HPLC

目的 :考查山东 4种菟丝子中槲皮素与山萘酚含量的差异。方法 :采用高效液相色谱法。结果 :山东 4种菟丝子中槲皮素与山萘酚的含量均较低 ,前者含量为 0 .0 0 0 2 %～ 0 .0 5 0 2 % ,后者为 0 .0 0 0 2 %～ 0 .0 2 0 5 %。其中菟丝子、金灯藤和啤酒花菟丝子中槲皮素含量明显高于山萘酚 ;而南方菟丝子正相反 ,槲皮素含量低于山萘酚。槲皮素与山萘酚的含量不仅与菟丝子的品种有关 ,而且与其寄生的植物有关。结论 :HPLC法可以作为菟丝子类药材质量评价的依据。

Objective: To explore the differences among contents of quercetin and kaempferol in four kinds of Semen Cuscutae in Shandong province. Methods : Quercetin and kaempferol are determined by HPLC. Results : The contents of both quercetin and kaempferol are lower in four kinds of Semen Cuscutae. The former is within 0.0002% to 0.0502% and the latter varies from 0.0002% to 0.0205%, respectively. In seeds of Cuscuta chinensis Lam., C. japonica Choisy and C.lupuliformis Krocker, quercetin contents are much higher than kaempferol contents. while in seed of C. australis R.Br., by contrast, its quercetin content is lower than kaempferol. The contents of quercetin and kaempferol have close relation with both the variety and the hostplant. Conclusion : HPLC can be used for the quality evaluation of Semen Cuscutae.