胆固醇对离体培养胰岛素细胞活性的损害作用

Damage of viability of insulin-secreting cells by cholesterol in vi tro

目的明确游离胆固醇对胰岛素细胞的作用。方法体外培养MIN6小鼠胰岛素细胞 ,待细胞生长融合后 ,加入不同浓度的游离胆固醇作用 2 4h ,或用同一浓度的游离胆固醇作用不同时间。采用MTT法检测细胞的存活情况。结果 2 5μmol/L胆固醇作用 2 4h可明显降低MIN6细胞的活性 ;1 0 0 μmol/L胆固醇作用 1 2h即可明显降低MIN6细胞的活性 ,作用 2 4h可导致MIN6细胞死亡。结论游离胆固醇可剂量依赖性和时间依赖性地降低胰岛素细胞的活性 ,提示血液中游离胆固醇浓度升高在胰岛

ObjectiveTo identify t he influence of cholesterol on insulin-secreting cells.Methods MIN6 mouse insulin cells were cultured in vitro. After confluence of cells, on one hand, free cholesterol in series concentrations was added to cultu re medium to act for 24 hours, and on the other hand, free cholesterol in same c oncentration was added to culture medium to act for different periods. MTT test was used to evaluate the viability of MIN6 cells.Results25μmol/L free cholesterol significantly decreased the viability of MIN6 c ells after 24 hours and 100μmol/L free cholesterol significantly decreased the viability of MIN6 cells after 12 hours and induced cells death after 24 hours.Conclusions Free cholesterol decreases viability of MIN6 cells in a dose-dependent and time-dependent manner, and it is indicated that elevated free cholesterol concentration in blood may be one factor involved in the dysfunction of pancreatic β-cells.