NF-κB在糖尿病大鼠肾脏和血液单个核细胞中的表达

NF-κB expression in the kidney and blood mononuclear cell of diabetic rats

探讨NF κB在糖尿病肾病 (DN)发病机制中的作用。应用电泳迁移率实验和超迁移率实验检测了 12周STZ—糖尿病大鼠血液单个核细胞和肾组织提取物中NF κB的表达及组成NF κB的亚单位。发现糖尿病大鼠肾组织和血液单个核细胞NF κB的表达显著高于正常对照组 (P均 <0 0 5 ) ,进一步利用超迁移率实验证实血液单个核细胞NF κB由P50 和P6 5抗体组成 ,而肾组织NF κB仅P6 5抗体参与 ;丙丁酚干预后可下调肾和血液单个核细胞NF κB的表达 ,但与糖尿病组比较 ,差异仍有显著性 (P均 <0 0 5 )。提示糖尿病时存在NF κB的激活与持续高表达 ,NF κB可能通过调节参与DN发病机制中重要因子的表达 ,在DN发生过程中起关键作用 ;丙丁酚可以抑制DN时NF κB的表达。

To investigate the role of NF κB in pathogenesis of diabetic nephropathy. Electrophoresis mobility shift assay and supershift assay were used to analyze the expression of NF κB activation and its componentes in the renal tissue and the blood mononuclear cells of streptozotocin induced diabetic and control rats at the 12th week . Nuclear extracts prepared from the blood mononuclear cells and renal tissue in diabetic nephropathy rats ,both displayed stronger DNA binding activity comparing to the control. Probucol suppressed the NF κB activation in the nuclear extracts of the blood mobonuclear cells and the renal tissue. Conclusion The result showed that NF κB possibly regulated the expression of many important cytokine and played a key role in pathogenesis of diabetic nephropathy. Probucol could inhibit the activation of NF κB in diabetic nephropathy model.