外显子6基因多态与系统性红斑狼疮的研究

Study on association between genetic polymorphism of exon 6 and systemic lupus erythematosus

目的 探讨肿瘤坏死因子受体Ⅱ (TNFRⅡ )外显子 6基因多态与华东地区汉族人群系统性红斑狼疮(SLE)易感性的关系。方法 采用聚合酶链式反应和限制性片断长度多态 (PCR -RFLP)方法对 34例SLE患者和 66例健康对照进行TNFRⅡ外显子 6基因分型。结果 在SLE患者与健康对照之间 ,TNFRⅡ外显子 6R等位基因频率(35 3 % /2 1 2 % ;OR =2 0 3 ,95 %可信限 :1 0 1～ 4 0 8;χ2 =4 63 ,P =0 0 31 )与基因型频率 (67 6 % /37 9% :OR =3 43 ,95 %可信限 :1 32～ 9 0 6 ;χ2 =7 97,P =0 0 0 5)均不同。结论 TNFⅡ外显子 6基因多态可能与华东地区汉族人群系统性红斑狼疮发病有关 。

Objective To make sure whether there is any association between genetic polymorphism of tumor necrosis factor receptor Ⅱ (TNFⅡ) exon 6 and systemic lupus erythematosus (SLE).Methods PCR-RFLP was used.A population-based study was carried out including 34 SLE patients and 66 health controls.Results The TNFⅡ exon6 R allele frequency of cases is significantly different from that of controls(35.3 %vs 21.2%; OR =2.03, 95%CI:1.01-4.08;χ\+2=4 63, P =0.031).The subjects with TNFⅡ exon6 R allele tend to be more susceptible to SLE than those without.Conclusion The subjects with TNFⅡ exon6 R allele tend to be more susceptible to SLE than those without.