亚洲型和西方型多发性硬化髓磷脂少突胶质细胞糖蛋白微卫星等位基因多态性的比较

Comparison of myelin oligodendrocyte glycoprotein microsatellite allelic polymorphisms in Asian and Western types of multiple sclerosis

目的 :比较亚洲型和西方型髓磷脂少突胶质细胞糖蛋白 (MOG)基因的多态性与比较亚洲型和西方型多发性硬化 (MS)患者。方法 :将 85例MS患者分为亚洲型 (4 0例 )和西方型 (4 5例 ) ,用微卫星法测定其MOG等位基因的多态性 ,并与 87例健康者 (对照组 )相比。结果 :MOG11/ 12等位基因与各型MS无关 (P >0 .0 5 ) ;亚洲型MSMOG5 1/ 5 2的 2 18bp等位基因频率显著高于西方型 ,西方型MS患者 2 18bp等位基因的频率显著低于对照组(P均 <0 .0 0 1) ;西方型MSMOG5 1/ 5 2的 2 2 2bp等位基因频率显著高于亚洲型和对照组 (P <0 .0 0 1～ 0 .0 5 ) ;亚洲型、西方型MS患者MOG5 1/ 5 2的 2 2 6bp等位基因频率均显著高于对照组 (P <0 .0 0 1～ 0 .0 0 5 )。 结论 :亚洲型和西方型是MS不同的临床亚型 ;MOG5 1/ 5 2的 2 2 2bp等位基因与西方型MS相关 ,与亚洲型MS无关 ;MOG5 1/ 5 2的 2 2

Aim: To explore the association of myelin oligodendrocyte glycoprotein (MOG) gene polymorphisms with multiple sclerosis (MS).Methods: A total of 85 patients of MS were allocated into Asian type(40 cases) and Western type(45 cases) . The MOG allelic polymorphisms of MS patients were detected by fluorescence labelling of PCR products and automated DNA sequencer. A total of 87 healthy unrelated individuals. Results: Asian type MS showed a significantly higher sex ratio(female/male), higher age of onset, and higher expanded disability status scale scores than those of Western type MS (all P <0.001). MOG11/12 alleles were not associated with Asian or Western types of MS( P >0.05). The frequency of 218 bp allele of MOG51/52 in Asian type MS was significantly higher than that in Western type MS ( P < 0.001 ). The frequency of 218 bp allele of MOG51/52 in Western type MS was markedly lower than that of healthy control ( P <0.001). The frequency of 222 bp allele in bothWestern type MS was significantly higher than that in Asian type MS and that of healthy control ( P <0.001～0.05). The frequency of 226 bp allele of MOG51/52 in Asian type MS and Western type MS was increased significantly compared with that of healthy control( P <0.001～0.005).Conclusion: Asian and Western types are 2 distinct subtypes of MS; the 222 bp allele of MOG51/52 is associated with Western type MS but not Asian type MS; the 226 bp allele of MOG51/51 may be a susceptical gene to MS.