提高高羊茅愈伤组织诱导率的研究

The research of improving the inductivity of callus of Festuca arundinacea

用不同浓度的2,4-D,不同种类的培养基,对3个不同品种高羊茅成熟种子进行愈伤组织诱导,结果表明:这3个高羊茅品种对2,4-D均不敏感,诱导浓度需高于一般禾本科诱导浓度2mg/L;3种不同培养基MS、CC、NB中,CC培养基为最适培养基。此外,本实验还对外植体处理方法进行了改进,将胚乳切除后,愈伤组织诱导率大大提高,愈伤组织质量也有很大程度改善。

Using different concentrations of 2,4D and different culture media, the callus induction experiment of three species of Festuca arundinacea was conducted, The results indicated that all of the three species of Festuca arundinacea was not sensitive to 2,4D, the concentrations of 2,4D for callus inducing was higher than other gramineous plants; CC is the optimal medium in all of the three media tested. In addition, this experiment also improved the treatment method of the explant. The induction frequency and quality of the embryonic calli had been greatly improved after excising endosperm.