鱼类细胞核移植研究进展及前景展望

Progress and prospect of nuclear transplantation in fish

细胞核移植技术是目前生物技术领域研究的热点之一,其科学意义和应用价值重大。本文介绍了中国和世界上鱼类细胞核移植的研究成果及其最新进展;综述了鱼类细胞核移植的基本程序、方法和影响因素,包括胞质受体的准备、核供体的制备、核移植和核质杂种的培养。并对鱼类细胞核移植的应用、现存问题及应用前景进行了讨论。

Presently, one of the hot spots in the fields of biotechnique is nuclear transplantation, which is of great importance in the field of bioscience.This review briefly introduces the progress of nuclear transplantation on fish. It describes the main methods and procedures used in nuclear transplantation, including preparation of cytoplast receptor and nuclear, nuclear transplanting and culture of the cytoplastnuclear hybrid. Some problems existing in this area were discussed. The application of the methods at present and in the future was also summarized.