pTet-On鼻咽癌SUNE1细胞系的建立

Establishment of pTet-On Nasopharyngeal SUNE1 Cell Line

目的:建立受四环素衍生物诱导表达外源基因的SUNE1细胞系,为研究特定基因定量表达在鼻咽癌的发生、转移及药物干预中的作用提供理想的细胞模型。方法:脂质体介导转染pTet-On质粒于鼻咽癌细胞系SUNE1,G418筛选得到稳定克隆,再瞬间转染pTRE-Luc,DOX诱导后检测光素酶(Luciferase)活性得到高水平诱导,低背景表达的SUNE1细胞系。结果:筛选得到的SUNE1细胞系受四环素衍生物诱导后能够低背景、高水平的表达。

Objective:To establish the SUNEl cell line induced by tetracylin and doxycline,and provide a useful in vitro expression model for the study of gene expression on the formation, metastasis and drug intervention of nasopharyngeal carcinoma. Methods: SUNE1 cells were transfected with pTet - On plasmid by lipofectamine, then the stably expressing clone was established by G418 screen. After the pTRE - Luc report plasmid was trasfected into the clone , the luciferase activity was detected to obtain clones that Luc expressed in high level and low background. Result: the clone obtained can express in high level and low background.