摘要
介绍了一种应用二阶导数紫外光谱法测定尿和血浆中华法林的新方法。尿和血浆用甲醇/乙酸缓冲溶液稀释后,通过PT-C18柱进行固相萃取,用氯仿洗脱。洗脱液用NaOH溶液反提,测定NaOH溶液的二阶导数光谱,用328nm波长处的吸光度进行定量。尿中华法林的检出限为1μg·ml^(-1),血浆为3μg·ml^(-1)。该方法可用于大剂量中毒检测,亦可用于微量血药浓度检测。
The 2nd-derivative UV-spectrometry was applied as a new method to the determination of warfarin in human urine and blood plasma. The sample was diluted with methanol and acetate buffer. Warfarin in the sample solution was separated on PT-C18 column by solid-extraction, and eluted from the column with chloroform. Warfarin was then stripped from chloroform with NaOH solution, and determined by UV-spectrometry at 328nm. The detection limit of warfarin in urine was found to be 1. 0μg · ml-1 and in blood plasma to be 3μg · ml-1.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2003年第5期293-294,296,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
