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四色法流式细胞术分析正常人T细胞亚群中IFN-γ和IL-4的表达 被引量:2

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摘要 目的 :研究IFN γ和IL 4在正常人T淋巴细胞亚群中的表达情况。方法 :5 0例正常人的外周血在PMA、ionomycin和BFA存在的条件下在体外短期培养 ,进行细胞膜表面抗原和细胞内细胞因子染色 ,再用流式细胞仪对CD3 +、CD3 +CD8+、CD3 +CD8-细胞内的IFN γ和IL 4进行测定。并对分析方法中的一些测定条件进行了探讨。结果 :正常人 9 89%~ 3 3 3 9%的CD3 +CD8 细胞为Th1细胞 ,0 64 %~ 6 87%为Th2细胞 ,14 0 0 %~63 76%的CD3 +CD8+细胞为Tc1细胞 ,0 92 %~ 5 44 %为Tc2细胞 ;Th1、Tc1细胞百分比明显多于Th2、Tc2细胞 ,并且Tc1细胞百分比比Th1细胞多 ,而同时分泌两种细胞因子的Th0、Tc0细胞均较少。结论 :T淋巴细胞各个亚群产生IFN γ和IL 4的能力明显不同且正常人分泌细胞因子的能力有较大的个体差异。
出处 《实用医学杂志》 CAS 2003年第5期560-562,共3页 The Journal of Practical Medicine
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