Ⅳ型胶原与层粘蛋白在皮肤角质形成细胞肿瘤中的表达

Expression of collagen type Ⅳ and laminin in keratinocyte-derived tumors of the skin

目的 :探讨皮肤角质形成细胞肿瘤中 型胶原和层粘蛋白的表达及其与肿瘤转移的关系。方法 :用 ABC免疫组化技术观察 48例皮肤鳞状细胞癌、40例基底细胞癌及 1 5例角化棘皮瘤手术切除标本。结果 : 型胶原和层粘蛋白在高分化或无淋巴结转移的鳞状细胞癌中的表达明显高于低分化或有淋巴结转移者 (P<0 .0 1 )。基底细胞癌中 型胶原和层粘蛋白的表达类似于鳞状细胞癌 (P>0 .0 5) ,但其表达明显高于有转移的鳞状细胞癌 (P<0 .0 1 )。角化棘皮瘤中 型胶原和层粘蛋白的表达一般呈连续性 ,未见明显缺乏。结论 :皮肤癌的癌分化、淋巴结转移与

Objective:To investigate the expression of collagen type Ⅳ(Col Ⅳ)and laminin (LN) in keratinocyte derived tumors of the skin and the relationship between their expression and tumor metastasis Methods:Expression of Col Ⅳ and LN was observed by ABC immunohistochemical technique in 48 cutaneous squamous cell carcinomas (SCCs),40 basal cell carcinomas (BCCs) and 15 keratoacanthomas (KAs) Results:The immunostaining of Col Ⅳ and LN was distributed along tumor nests or cells in fragmentary,disruptively linear or continuously linear patterns Compared with the poorly differentiated SCCs or SCCs with lymph node metastasis,the expression of Col Ⅳ and LN in well differentiated SCCs or SCCs without positive metastasis was upregulated ( P <0 01) Though the expression of Col Ⅳ and LN in BCCs was similar to that in SCCs ( P >0 05),it was much higher than that in SCCs with lymph node metastasls ( P <0 01) Expression of Col Ⅳ and LN was generally continuous and there was no obviously absent in KAs Conclusion:Expression of Col Ⅳ and LN relates to the differentiation and lymph node metastasis in skin carcinoma