急性氧中毒小鼠血清细胞因子水平的变化

Changes of Serum Level of Cytokines in Mice after Acute Oxygen Toxicity

为探讨细胞因子在急性氧中毒中的作用和意义,用酶联免疫吸附法检测急性氧中毒后1、6和12 h小鼠血清IL-1β、IL-6、IL-10的质量浓度。结果显示,急性氧中毒1、6、12 h时小鼠血清IL-1β的质量浓度均高于正常对照组,有显著或非常显著性差异(P<0.05或P<0.01);急性氧中毒6、12 h时血清IL-6的质量浓度均高于正常对照组,有显著或非常显著性差异(P<0.05或P<0.01);急性氧中毒后血清IL-10的质量浓度无显著性变化。提示急性氧中毒早期存在促炎症介质和抗炎症介质之间的失衡,这可能是急性氧中毒发生和发展的重要因素。

The objective was to investigate the role and significance of cytokines in mice after acute oxygen toxicity. The serum level of IL-1β, IL-6 and IL-10 in mice at the 1 h, 6 h and 12 h after acute oxygen toxicity was measured by enzyme-linked immuno-sorbent assay. The serum level of IL-1β at the 1 h, 6 h, 12 h was higher than the normal control group(P<0.05 or P<0. 01). The serum level of IL-6 in mice at the 6 h, 12 h was higher than normal control group(P<0.05 or P<0.01). There were no significant changes in the serum level of IL-10. The results indicated that there was an imbalance between inflammatory and anti-inflammatory mediators during earlier period after acute oxygen toxicity, which might be the important factor in the onset and development of the acute oxygen toxicity.