摘要
AIM: Cyclooxygenase-2 is involved in a variety of important cellular functions, including cell growth and differentiation,cancer cell motility and invasion, angiogenesis and immune function. However, the role of cyclooxygenase-2 as an angiogenic factor in colorectal cancer tissue is still unclear.We investigated the relationship between cyclooxygenase2 and angiogenesis by analyzing the expression of cyclooxygenase-2 in colorectal cancer tissue, as well as its association with vascular endothelial growth factor (VEGF)and microvascular density (MVD).
METHODS: The expression of cydooxygenase-2, VEGF, as well as MVD was detected in 128 cases of colorectal cancer by immunohistochemical staining. The relationship between the cydooxygenase-2 and VEGF expression and MlVD was evaluated.Our objective was to determine the effect of cyclooxygenase2 on the angiogenesis of colorectal cancer tissue.
RESULTS: Among 128 cases of colorectal cancer, 87 were positive for cyclooxygenase-2 (67.9 %), and 49 for VEGF (38.3 %), respectively. The microvessel counts ranged from 23 to 142, with a mean of 51.7 (standard deviation, 19.8). The expression of cydooxygenase-2 was correlated significantly with the depth of invasion, stage of disease, metastasis (lymph node and liver), VEGF expression and MlVD. Patients in T3-T4, stage Ⅲ-ⅣV and with metastasis had much higher expression of cydooxygenase-2 than patients in T1-T2, stage Ⅰ-Ⅱ and without metastasis (P＜0.05). The positive expression rate of VEGF (81.6 %) in the cyclooxygenase-2 positive group was higher than that in the cyclooxygenase-2 negative group (18.4 %,P＜0.05). Also, the microvessel count (56±16) in cyclooxygenase-2 positive group was significantly higher than that in cyclooxygenase-2 negative group (43±12, P＜0.05). The microvessel count in tumors with positive cyclooxygenase-2and VEGF was the highest (60±18, 41-142, P＜0.05), whereas that in tumors with negative cyclooxygenase-2 and VEGF wasthe lowest (39±16, 23-68, P＜0.05).
CONCLUSION: Cyclooxygenase-2 may be associated with tumor progression by madulating the angiogenesis in colorectal cancer tissue and used as a possible biomarker.
AIM:Cyclooxygenase-2 is involved in a variety of important cellular functions,including cell growth and differentiation, cancer cell motility and invasion,angiogenesis and immune function.However,the role of cyclooxygenase-2 as an angiogenic factor in colorectal cancer tissue is still unclear. We investigated the relationship between cyclooxygenase- 2 and angiogenesis by analyzing the expression of cyclooxygenase-2 in colorectal cancer tissue,as well as its association with vascular endothelial growth factor (VEGF) and microvascular density (MVD). METHODS:The expression of cyclooxygenase-2,VEGF,as well as MVD was detected in 128 cases of colorectal cancer by immunohistochemical staining.The relationship between the cydooxygenase-2 and VEGF expression and MVD was evaluated. Our objective was to determine the effect of cyclooxygenase- 2 on the angiogenesis of colorectal cancer tissue. RESULTS:Among 128 cases of colorectal cancer,87 were positive for cyclooxygenase-2 (67.9 %),and 49 for VEGF (38.3 %),respectively.The microvessel counts ranged from 23 to 142,with a mean of 51.7 (standard deviation,19.8).The expression of cyclooxygenase-2 was correlated significantly with the depth of invasion,stage of disease,metastasis (lymph node and liver),VEGF expression and MVD.Patients in T3-T4,stage III-IV and with metastasis had much higher expression of cyclooxygenase-2 than patients in T1-T2,stage I-II and without metastasis (P<0.05).The positive expression rate of VEGF (81.6 %) in the cyclooxygenase-2 positive group was higher than that in the cyclooxygenase-2 negative group (18.4 %, P<0.05).Also,the microvessel count (56±16) in cyclooxygenase-2 positive group was significantly higher than that in cyclooxygenase-2 negative group (43±12,P<0.05).The microvessel count in tumors with positive cyclooxygenase-2 and VEGF was the highest (60±18,41-142,P<0.05),whereas that in tumors with negative cyclooxygenase-2 and VEGF was the lowest (39±16,23-68,P<0.05). CONCLUSION:Cyclooxygenase-2 may be associated with tumor progression by madulating the angiogenesis in colorectal cancer tissue and used as a possible biomarker.
基金
Hubei Province Natural Science Foundation,No.2000J054
