细胞因子、IgE及一氧化氮在小儿分泌性中耳炎中耳积液的表达

Expression of cytokine and immunoglobulin E and NO in otitis media with effusion in children

目的 :探讨细胞因子、IgE及一氧化氮 (NO)在小儿分泌性中耳炎的发生及转归中的作用。 方法 :检测 70例 (1 2 9耳 )分泌性中耳炎患儿 (患儿组 )血浆及中耳积液中细胞因子、IgE及NO含量 ,并以 30例健康儿童作对照。结果 :患儿组白细胞介素 (IL 2、4、6、8、1 0 )、肿瘤坏死因子 α(TNF α)、IgE及NO在中耳积液中含量较血浆中高 (P <0 .0 1 ) ;患儿组的血浆含量较对照组高 (P <0 .0 5 )。病程短者中耳积液中IL 2、IL 4的含量较病程长者高 ;而病程长者IL 6、IL 8、IL 1 0、TNF α、IgE及NO的表达较病程短者增加 (均P <0 .0 5 )。结论 :在儿童分泌性中耳炎的血浆及中耳积液中细胞因子、IgE及NO表达增强 ,细胞因子参与介导中耳局部的炎性反应 ,调节局部的免疫反应 。

Objective:To study the role of cytokine, IgE and NO in the pathogenesis of children with otitis media with effusion.Method:The content of cytokines and Ig E in blood plasma and middle ear effusion were measured in 70(129 ears) children cases of otitis media with effusion and 30 normal subjects respectively.Result:The levels of cytokines, IgE and NO in OME were remarkably higher than those in blood plasma. The levels of serum IL 2,IL 4,IL 6,IL 8,IL 10,TNF α,NO and IgE in the OME group were obviously higher than those in the normal control group. It revealed that the shorter the course, the higher the levels of IL 2 and IL 4,and the longer the course, the higher the levels of others.Conclusion:These data suggest that cytokines act as mediators of inflammation and regulators of the immune response, which may have an effect on the outcome of OME.