重离子^(12)C^(6+)对^(60)Coγ射线诱发淋巴细胞微核效应的比较研究

A comparative study on micronuclei production induced by carbon ion versus ^(60)Coγ-rays in human peripheral blood lymphocytes

目的 比较重离子束1 2 C6+对60 Coγ射线照射人离体血诱发淋巴细胞微核的效应。方法 用60 Coγ射线和地面加速器产生的重离子1 2 C6+(平均LET为 36 70keV μm) ,不同剂量照射离体人血 ,用CB微核法观察双核淋巴细胞的微核 ,计算重离子束1 2 C6+对60 Coγ射线的相对生物效能。结果 在 0～ 6Gy剂量范围内 ,重离子束1 2 C6+对60 Coγ射线的相对生物效能在 4 1 9～ 1 78之间 ,平均为 2 56。结论 重离子束1 2 C6+比60

Objective\ To compare the effectiveness of carbon ion versus 60 Co γ rays in producing micronuclei in human peripheral blood lymphocytes. Method\ Heparinized whole blood samples were exposed to carbon ion and 60 Co γ rays,respectively.Micronuclei in binuclear lymphocytes (CB method) were scored.Relative biological effectiveness (RBE) values were caluclated. Results\ RBE values of carbon ion decreased with increasing dose (ranged from 1 78 to 4 19 Gy).The average RBE value is 2 56. Conclusion\ Carbon ion has higher biological effectiveness in inducing micronuclei than 60 Co γ\|rays do.\;