固本止咳膏质量标准的研究

Studies on the Quality Standard of Guben Zhike Electurary

目的 :建立固本止咳膏质量控制的方法。方法 :采用薄层色谱法对该药的土垄大白蚁巢、五味子、淫羊藿和黄芪进行定性鉴别 ;采用高效液相色谱法测定该药五味子甲素的含量。液相色谱法条件为 :色谱柱Shim PackCLS ODS柱 (15 0×6 0mm) ;流动相∶甲醇 水 (85∶15 ) ,流速 1ml·min- 1 ,检测波长 2 5 4nm。结果 :该药薄层层析斑点清晰集中 ,含量测定五味子甲素在 0 0 6～ 0 5 4 μg范围内具有良好线性关系 ,平均回收率为 96 0 % ,RSD =2 15 % (n =6 )。结论 :本方法灵敏准确、重现性好、专属性强 ,可作为固本止咳膏的质量控制标准。

Objective:To establish the method for quality control of Guben Zhike Electuary.Methods:Nidus Macrotermis,Fructus Schisandrae,Herba Epimedii and Radix Astragali were identified by TLC.The content of deoxyschisandrin was determined by HPLC.Conditions of HPLC were:Shim\|Pack CLS\|ODS column(150×6 0mm)with detection wavelength at 254nm.The mobile phase consisting of a mixture of methanol\|water(85∶15).The flow rate was 1 ml·min -1 .Result:The spots of TLC were clear and concentrative.The linear regression for Deoxyschisandrin was obtained over the range of 0\^06～0\^54μg.The average revovery was 96\^0%, RSD =2\^15%( n =6).Conclusion:The methods are sensitive,accurate,reproducible and exclusive.It can be use for quality control of Guben Zhike Electuary.