摘要
目的 :研究复方中药———复方三根制剂对多药耐药 (MDR)细胞株的逆转作用。方法 :应用MTT比色法 ,观察复方三根制剂对MDR细胞的逆转作用。运用流式细胞仪 (FCM) ,测定其对耐药细胞积聚和外排阿霉素 (ADR)的影响 ,以及对耐药株细胞表达p gp的影响。结果 :复方三根制剂可部分恢复K5 6 2 ADR和K5 6 2 VCR耐药细胞对ADR的敏感性 ,而对VCR抗药性的逆转作用不明显 ;可增加K5 6 2 ADR和K5 6 2 VCR耐药细胞内ADR的积聚 ,并对外排ADR有一定影响 ;可部分下调p gp的表达。结论 :复方三根制剂可部分逆转耐药细胞的抗药性。
Obsjective\ To observe the reversal effect of Fufang Sangen Mixture(FFSG) in multidrug resistant(MDR)cell lines K562/ADR and K562/VCR.Methods\ We used MTT assay to observe the mixture's reversal effect.Then,we determined the effect of FFSG on intracellular adriamycin(ADR) accumulation,drug efflux and the expression of p\|gp by flowcytometry(FCM).Results\ 10mg/ml,20mg/ml of FFSG can reverse the K562/ADR cell's resistance to adriamycin to some degree and 20mg/ml FFSG can also partly reverse the K562/VCR cell's resistance to adriamycin.10mg/ml,20mg/ml concentration of FFSG can enhance the intracellular adriamycin accumulation in K562/ADR and K562/VCR cell lines.FFSG also has the effect on the efflux of intracellular adriamycin and decreasing the expression of p\|gp in MDR cell lines.Conclusion\ FFSG can partly reverse the resistance of MDR cell lines.
出处
《中国实验方剂学杂志》
CAS
2003年第4期26-30,共5页
Chinese Journal of Experimental Traditional Medical Formulae
