中药SSY-B2促进中枢神经系统损伤后轴突再生的分子机制

Effect of SSY-B2 on regeneration of central nervous system of rats with bilateral fornix/fimbria transaction

目的观察中药SSY B2对中枢神经系统神经再生的促进作用。方法成年雄性SD大鼠随即分为 6组 :假手术组、模型组、阳性对照药脑复康治疗组 ( 0 5 6g纯药 kg)、SSY B2小剂量治疗组 ( 1 5g生药 kg)、SSY B2中剂量治疗组 ( 3 g生药 kg)、SSY B2大剂量治疗组 ( 6g生药 kg)。大鼠行双侧隔海马穹窿伞离断术。术后给予SSY B2等试验用药 6周。在行为学测试结束后 ,采用免疫组织化学方法 ,在大脑有关区域测定生长相关蛋白 43 (GAP 43 )、硫酸软骨素蛋白多糖 (CSPG)。结果伤口周围GAP 43的阳性细胞数假手术组与模型组相比无显著性差异 (P >0 0 5 ) ,SSY B2小剂量能显著促进损伤后GAP 43的表达 (与模型组相比 :P <0 0 0 1)。损伤后顶叶皮层有CSPG表达 ,假手术组和模型组密度分别为 ( 5 6 43± 5 9 6)、( 116 3 6± 10 5 61) ,SSY B2小、中剂量能抑制损伤后CSPG的表达 (和模型组相比分别为P <0 0 5 ,P <0 0 1)。结论SSY B2小剂量和脑复康能显著促进损伤后GAP 43的表达 ,抑制CSPG表达 ,起到促进中枢神经系统 (CNS)神经再生的作用 ,而轴突再生可能在某种程度上与损伤后的结构和功能修复有关。

ObjectiveTo study the effect of SSY-B2 on regeneration of central nervous system of rats with bilateral fornix/fimbria transaction.MethodsMale adult SD rats were divided randomly into 6 groups as sham group,model group, positive control agent piracetam group, SSY-B2 low dosage(1.5g crude drug/kg) group, medium dosage(3g crude drug/kg) group and high dosage(6g crude drug/kg) group.The bilateral fornix/fimbria transection in the rats were carried out. After operation, drugs were fed introgastrically to each group respectively for 6 weeks. The immunoreactive products of growth-associated protein-43(GAP-43 )and chondroitin sulfate proteoglycans(CSPG) in defined areas were measured using immunohistochemical methods. ResultsThere was no difference in number of cells expressing GAP-43 between the model group and sham group (P>0.05) , but that in low dosage group increased compared with the model group (P<0.001). The CSPG in parietal lobes after lesion expressed,which was in sham group and model group(sham group and model group respectively, 56.43±59.6,116.36±10.561), and SSY-B2 in low and medium dosage inhibited its expression compared with the model group (P<0.05, P<0.01). Conclusions SSY-B2 can enhance the expression of GAP-43 and inhibit the expression or deposition of CSPG, promote axonal regeneration in the CNS, and thus structural repair and functional restoration in certain degree.