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睡眠剥夺对大鼠血清髓鞘碱性蛋白和海马超微结构的影响 被引量:1

Effects of sleep deprivation on the serum level of myelin basic protein and ultrastructure of hippocampus in rats
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摘要 目的 观察大鼠睡眠剥夺 (SD)后的脑损害情况。 方法 采用小平台水环境法建立SD模型 ,以大平台组 (TC)和正常笼养组 (CC)作为对照组 ,采用酶联免疫吸附法测定髓鞘碱性蛋白(MBP)含量、采用双抗体放射免疫法测定皮质醇含量。电镜观察海马神经细胞超微结构变化。 结果 与 CC和 TC组比较 ,SD 1d、3d和 5 d后血清皮质醇水平均增高 ,差异有显著性意义 ;SD 5 d后血清 MBP含量增高 ,差异有显著性意义 ,SD 1d、3d水平无显著性差异。TC组与正常对照组比较 ,血清皮质醇增高而 MBP水平无显著性差异。电镜下观察 SD 5 d组 CA3区锥体细胞形态不规则 ,结构疏松 ,髓鞘板层分离、线粒体肿胀、空泡变性。 结论 长时间 SD可能引起脑器质性损害。 Objective To investigate harmful effects of sleep deprivation (SD) on brain in rats. Methods Sleep deprivation rat model was established by housing rats on small platform in a water tank. Thirty Sprague-Dawley rats were divided into 5 groups: normal cage control group (CC), tank control group (TC, housing rats on large platforms in water tank), sleep deprivation 1 d group(SD 1 d), SD 3 d group and SD 5 d group. Blood samples were collected immediately after experiment. Serum level of cortisol was measured by double antibodies radioimmunoassay and myelin basic protein (MBP) measured by enzyme linked immuno-absorbent assay. Ultrastructure of hippocampus were observed by electromicroscope. Results Compared with CC and TC groups, serum levels of cortisol of SD 1 d, 3 d, 5 d groups and serum level of MBP of SD 5 d group were significantly higher. Serum level of cortisol of TC group was higher than that of CC group. In section of hippocampus from rats of SD 5 d group, form anomalous,structure rarefaction, and intercellular space augmentation of pyramidal cells and myelinic degeneration as lamellar structure, swelling and vacuolation of mitochondrion were observed. Conclusion The elevation of serum MBP level and ultrastructure changes in hippocampus of 5 d SD group suggested that prolonged SD may induce brain damages in rats.
出处 《中华航空航天医学杂志》 CSCD 2003年第2期107-109,F002,共4页 Chinese Journal of Aerospace Medicine
基金 兰州军区医药卫生科研计划项目 (L XH0 2 -0 3 )
关键词 睡眠剥夺 应激 氢化可的松 髓磷脂碱性蛋白质类 显微镜检查 电子 Sleep deprivation Stress Hydrocortisone Myelin basic proteins Microscopy, electron
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