褪黑素对人前列腺癌PC_3细胞的增殖抑制作用

Inhibition in in vitro proliferation human prostate cancer PC_3 cells induced by melatonin

目的 体外研究褪黑素 (melatonin ,MLT)对人前列腺癌PC3细胞的增殖抑制作用及与 5 Fu联合用药的的影响。方法 用细胞计数法绘制细胞生长曲线、MTT比色法测定细胞活力、改良对硝基酚法测定酸性磷酸酶 (ACP)、改良二苯胺法测细胞内DNA含量及考马斯亮蓝法测定蛋白含量 ,并观察与 5 Fu联合用药对PC3细胞的影响。结果 MLT对PC3、CoLo2 0 5、K562和HeLa细胞的IC50 分别为 (1 2 0±0 30 )、(1 2 7± 0 1 2 )、(1 60± 0 1 6)和 (2 2 5± 0 1 1 )mmol·L- 1 ,MLT对上述细胞增殖的抑制呈时间和浓度依赖性 ,MLT减少PC3细胞的蛋白、DNA含量和降低酸性磷酸酶活性 ,MLT与 5 Fu 0 5mg·L- 1 联合用药有协同作用。结论 MLT对体外培养的肿瘤细胞的增殖有抑制作用 ,与 5

AIM To explore the inhibition in in vitro p roliferation human prostate cancer PC 3 cells induced by melatonin and the effe ct of combination of melatonin and 5-Fu. METHODS Cell growth cu rve, MTT assay, and acid phosphatase(ACP)activity were used to determine cell proliferation. Coomassie brillient blue assay and modified diphenylamine assay w ere used to measure the content of protein and DNA in PC 3 cells. ACP activity was measured by modified phenyl phosphat e method of kind and king and modified method of lowry. Co-administration effec t of MLT and 5-fluorouracil(5-Fu)on PC 3 cell proliferation was determined by MTT assay. RESULTS The IC 50 of MLT for inhibiting PC 3, CoLo205, K562, and HeLa cell proliferation was(1 20±0 30),(1 27±0 12) ,(1 60±0 16)and(2 25±0 11)mmol·L -1 , respectively. And MLT inhib ited all above cells proliferation in a concentration-dependent manner. The protein and DNA content and ACP activity in PC 3 cell treated with 2 mmol·L -1 MLT for 96 h were redu ced 58 77%, 54 97% and 89 24%, respectively. When PC 3 cells were treated wi th MLT in combination with 5-Fu 0 5 mg·L -1 , the inhibition rate enhance d. CONCLUSION MLT inhibited PC 3, CoLo205, K562, and HeLa cell p roliferation, and reduced the content of protein and DNA and ACP activity in PC 3 cell. MLT and 5-Fu co-administration enhanced the inhibition rate of PC 3.er.Theprotein andDNAcontentandACPactivityinPC3 celltreated with 2mmol·L-1MLTfor 96hwerereduced 5 8 77% ,5 4 97%and 89 2 4% ,respectively .When PC3 cellsweretreatedwithMLTincombinationwith 5 Fu 0 5mg·L-1,theinhibitionrateenhanced . CONCLUSION MLTinhibitedPC3 ,CoLo2 0 5 , K5 62 ,