2个水稻花发育相关MADS-box基因的全序列cDNA克隆及结构分析

Cloning and Structural Analysis of the Full-length cDNA of Two Rice Flowering-related MADS-box Genes

一组具有MADS box结构域的转录因子在控制花器官的诱导与发育中起着重要作用.以水稻广陆矮4号(OryzasativaL.Guang Lu AiNo.4)幼穗总RNA为模板,根据MADS box保守区的序列设计简并性引物,利用3′ RACE的方法获得了2个新的水稻花发育相关MADS box基因,分别命名为FDRMADS3和FDRMADS4;并利用5′ RACE的方法获得了该2个基因完整的cDNA序列,包括完整的编码区,5′ UTR和3′ UTR.它们编码的蛋白质都具有典型的MADS box结构域和半保守的K区,其与水稻中其他家族成员的MADS box结构域同源性高达90%以上,这说明它们都是典型的MADS box基因.

A group of regulatory factors containing the MADSbox domain plays an important role in controlling floral organ formation and development. In this study, total RNA from young inflorescence of riceGuangLuAi No.4 as template and a conservative DNA sequence of MADSbox to design primers were used, 2 new rice floweringrelated MADSbox gene cDNAs, named as FDRMADS 3 and FDRMADS4 respectively, were isolated by the 3'RACE methodology; and their fulllength cDNAs were obtained, including the whole open reading frame, 5'UTR and 3'UTR, by the 5'RACE methodology. Proteins encoded by these 2 genes contain a typical MADSbox domain that shows more than 90% identity to that of the other rice MADSbox proteins and a semiconservative Kbox, which means that FDRMADS3 and FDRMADS4 are typical MADSbox genes.