致敏小鼠肺组织eotaxin和TNF-α的表达及药物的影响

Expression of eotaxin mRNA and TNF-α mRNA in lung tissues of sensitized mice and modulation by anti-inflammatory drugs

目的 :建立测定肺组织趋化因子 eotaxin m RNA、α-肿瘤坏死因子 (TNF- α) m RNA表达的方法 ,观察抗炎药物对其表达的影响。方法 :采用半定量 RT- PCR法测定肺组织 eotaxin m RNA、TNF- α m RNA表达 ,并通过观察支气管肺灌洗液中白细胞总数和嗜酸性粒细胞的数目来验证 eotaxin和 TNF- α m RNA表达增多的功能。结果 :致敏小鼠抗原攻击后肺组织 eotaxin m RNA和 TNF- α m RNA表达与正常小鼠比较明显增多 ,支气管肺灌洗液中白细胞总数和嗜酸性粒细胞数目相应增加。抗炎药地塞米松对 eotaxin m RNA、TNF- α m RNA表达以及炎症细胞浸润均有明显抑制作用 ;受试的复方中药对 eotaxin m RNA表达以及炎症细胞浸润有明显抑制作用 ,对 TNF- α表达无明显影响。结论 :肺组织 eotaxin m RNA表达与嗜酸性粒细胞增多一致 ,表明半定量 RT- PCR测定肺组织 eo-taxin m RNA的方法可用于探索平喘药物的作用机制。

Objective: To establish determination methods of eotaxin mRNA and TNF-α mRNA expression in the lung tissue of mice. Methods: Eotaxin mRNA and TNF-α mRNA expressions were determined by semi-quantitative RT-PCR. The functional implications of eotaxin mRNA and TNF-α mRNA expression were examined by detecting the numbers of total leucocytes and eosinophils in bronchoalveolar lavage fluid(BALF). Results: Eotaxin mRNA and TNF-α mRNA expression in lung tissue,total numbers of leucocyte and numbers of eosinophil in BALF increased in sensitized mice compared with those in normal mice. Dexamethasone significantly but did not inhibite eotaxin mRNA and TNF-α mRNA expressions,and eosinophil infiltration in the lungs of the sensitized mice. A compound preparation of traditional Chinese medicine inhibited eotaxin mRNA and eosinophil infiltration, influenced TNF-α mRNA expression. Conclusion: Increased eotaxin mRNA expression in lung tissue is associated with eosinophil infiltration in BALF,which indicates that the methods of semi-quantitative RT-PCR may be useful to the study of the mechanism of antiasthmatic medicine.