14个板栗品种遗传多样性的RAPD分析

Genetic Diversity of 14 Chinese Chestnut (Castanea Mollissima BL.) Cultivars Using RAPD Marker

利用RAPD分子标记手段,研究板栗品种的遗传多样性。采用从100个随机引物筛选的20个引物对14个板栗品种的基因组DNA进行扩增,通过对140条谱带的聚类,分析了供试板栗品种的系统发育;运用特殊谱带,建立了板栗品种的分子检索表,结合扩增的特殊位点,提出了重点保存的板栗品种。

The genetic diversity of fourteen cultivars of Chinese chestnut was studied by RAPD molecular markers.with 20 primers selected from 100 arbitrary 10mer primers. Phylogenetic relationship was analyzed through cluster of bands in 140 amplified loci. Molecular checking index and conservation of key germplasms were put forward according to special bands.