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双氯芬酸对白血病K562细胞的影响及与阿霉素的协同作用

Effects of diclofenac on proliferation in leukemia K562 cells and synergism with adriomycin
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摘要 目的 :观察非甾体类抗炎药双氯芬酸及其与阿霉素联合用药对白血病K5 6 2细胞增殖和凋亡的影响。方法 :采用MTT比色法、流式细胞仪分析技术 ,检测双氯芬酸及其与阿霉素联合用药对K5 6 2细胞增殖和细胞周期分布、凋亡的影响。结果 :双氯芬酸呈剂量依赖性方式抑制K5 6 2细胞增殖 ,且可协同阿霉素增加其抗肿瘤细胞增殖效应 ;流式细胞仪分析表明单用双氯芬酸或阿霉素均可诱导细胞凋亡 ,但两者合用可使阿霉素的凋亡率从 (13.3± 1.0 ) %显著升高到 (6 8.0± 3.6 ) %。与细胞作用 4 8h后 ,双氯芬酸、阿霉素单用或合用均使细胞阻滞于G0 G1期 ,S期细胞明显减少。结论 :非甾体类抗炎药双氯芬酸能抑制白血病K5 6 2细胞的增殖并诱导其凋亡 ;双氯芬酸与阿霉素对K5 6 2细胞在抗增殖及促凋亡方面有协同作用。 AIM: To explore the effect of diclofenac on the proliferation in leukemia K562 cells and the synergism with adriomycin (ADM). METHODS: Cell viability and cell cycle were determined by MTT assay and flow cytometry. K562 cells were separately treated with different dosages of diclofenac, or ADM, or diclofenac+ADM in vitro. RESULTS: The cell proliferation was inhibited significantly by the diclofenac. The large the dosage, the stronger the action. ADM (25 mg·L -1 ) alone inhibited the growth of K562 cells, but this effect strengthened when diclofenac was added. Diclofenac or ADM alone elicited apoptotic changes, but ADM plus diclofenac obviously enhanced the number of apoptotic cells from 13.3 %± 1.0 % to 68.0 %± 3.6 %. After exposure to different concentrations of diclofenac (with or without ADM) for 48 h, K562 cells were accumulated in G_0/G_1 phase, and the cell number decreased in S phase. CONCLUSION: Diclofenac inhibits the proliferation and elicits apoptosis on the K562 cells. Diclofenac combined with ADM appears to interact in a synergistic manner.
出处 《中国临床药理学与治疗学》 CAS CSCD 2003年第4期408-410,共3页 Chinese Journal of Clinical Pharmacology and Therapeutics
关键词 药理学 双氯芬酸 白血病 阿霉素 协同作用 凋亡 pharmacology diclofenac leukemia adriomycin synergism apoptosis
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